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Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.

: While it started in futuristic science fiction, it gained broader colloquial popularity by the 1970s as a metaphorical way to advise patience or emotional control. When to Use It
The phrase emerged in the during the mid-20th century, a period deeply influenced by rapid developments in aviation and the "Space Age". 1. Cool Your Jets
: To stop someone from getting overly worked up or angry (e.g., "Cool your jets! It's not worth fighting over."). : While it started in futuristic science fiction,
: A classic alternative for advising patience. 1. Cool Your Jets
: To tell someone to wait when they are rushing a process (e.g., "Cool your jets—the results won't be ready for another hour."). Common Synonyms
or Take a chill pill : Modern, very informal ways to say the same thing.